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Original Articles

Simultaneous Multi-Wavelength UV-Visible Detection for HPLC Using a Diode-Array Spectrophotometer—A Versatile Software Package

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Pages 387-394 | Published online: 05 Dec 2006
 

Abstract

A BASIC program was developed to monitor absorbance simultaneously at various wavelengths using an HP8451A Diode Array Spectrophotometer linked to a HPLC system and equipped with a flow cell. The program measures a maximum of ten wavelengths and takes a maximum of 240 measurements for each wavelength, using only 16K of memory and a dual disc drive. The program includes the options of replotting using a different ordinate scale and saving the numerical data on disc.

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