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Abstract
A high performance liquid chromatography procedure using an internal carotenoid standard, echinenone, is described. The method uses a C-18, reverse-phase column, an isocratic solvent (acetonitrile:methylene chloride:methanol, 70:20:10) and requires only thirteen minutes. Most human plasmas gave seven distinct peaks; six carotenoids are identified. The sum of seven peaks is 90 percent or more of the total carotenoids in a total lipid extract.