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Abstract
The determination of thiols and disulfides independent of one another is accomplished through the use of dual electrode liquid chromatography/electrochemistry and N-ethylmaleimide. The method can be used to assess peak purity as well as to determine the oxidation state of an unknown thiol. By the addition of NEM, trace disulfides can be determined without interferences from glutathione or other thiols present in much higher concentrations in in the liver. This method is used for the detection of mixed disulfides in liver samples.