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Original Articles

Separation of Tocols by HPLC on an Amino-Cyano Polar Phase Column

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Pages 707-717 | Published online: 05 Dec 2006
 

Abstract

A method is described for separating α-, β-, γ and δ-tocopherols, their corresponding tocoraonoenols and tocotrienols, and plastochromanol-8 on a 5 μm amino-cyano HPLC column using hexane:tetrahydrofuran (94:6) as eluant. The detection limit for α-tocopherol using fluorescence excitation at 210 nm was 1 ng. Results of analysis on six seed oils are presented.

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