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Abstract
A method for the separation of serum phospholipid molecular species by reversed-phase high-performance liquid chromatography is described. Total serum phospholipid extracts were hydrolyzed with phospholipase C and benzoylated, and the resulting monoglyceride, diglyceride, and ceramide derivatives were separated by using gradient elution with detection at 230nm. Both human and rat serum phospholipid extracts were examined, and the use of this method for the evaluation of serum phospholipid metabolic alterations accompanying exposure to an environmental toxicant (polybrominated biphenyls) is described.