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Abstract
High performance preparative chromatographic systems are described which facilitate the isolation and purification of some naturally occurring, novel mycinamicins. Facile separation and excellent recovery of these macrolide antibiotics was obtained with a C-18 radially compressed Prep 500 cartridge using methanol/trifluoroacetic acid mobile phases. Semi-preparative chromatography on a C-8 reversed-phase sorbent incorporating a volatile ammonium acetate buffer was utilized in the final purification of an enriched mixture of intermediate components. Monitoring of isolated fractions with analytical HPLC provided direct support for the effectiveness of the preparative isolation.