Liquid Chromatography with Mixed Mobile Phases: Interpretation of the Model Chromatographic Data by Means of the Simple Linear Relationship

Abstract

A discussion is presented concerning the application of the simple linear relationships In K_s vs. In x and In K_s vs. x, where K_s is the solute distribution coefficient and x is the mole fraction of the more efficient eluting solvent in the mobile phase, for analysing LC data calculated for different chromatographic models. It is shewn that these linear relationshipo give a satisfactory representation of the LC data relating to none different models in a limited concentration region of the more efficient eluting solvent. Hence, it in difficult to select the LC nodel properly representing a given chromatographic system on the basis of the parameters obtained by fitting LC data to these linear relationships.