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Abstract
Optimization for minimum analysis time is demonstrated for HPLC separations of different degrees of difficulty. The separation of benzene and perdeuterobenzene can be performed la less than ten minutes, separation of benzene and monodeuterobenzene takes several hours.
The use of deuterium oxide and long microbore columns is not recommended since two-column recycle chroiaatography offers shorter analysis times than a long single-pass column.