Preliminary Studies for Interfacing Countercurrent Chromatography (CCC) with Fourier Transform Infrared (FT-IR) Spectrometry

Abstract

High speed countercurrent chromatography (CCC) and its use as an interface to a Fourier transform infrared (FT-IR) spectrometer are described. In this preliminary study the high solute-to-solvent ratios attainable with CCC are investigated, and infrared spectra are recorded of CCC fractions of aromatic components. The resulting infrared spectra of mg/mL concentration CCC fractions have very high signal-to-noise ratios, and no interference bands from the stationary phase (water/methanol) can be seen. Chloroform was used as the mobile phase. This study clearly demonstrates that a CCC/FT-IR spectrometry interface is feasible.