15
Views
28
CrossRef citations to date
0
Altmetric
Original Articles

Use of Coil Planet Centrifuge in the Isolation of Antibiotics

, &
Pages 2259-2280 | Published online: 13 Dec 2006
 

Abstract

In the course of isolating antibiotics from actinomycete fermentations, we have found the Ito Multi-Layer Coil Planet Centrifuge (CPC) can effect separations of such quality on crude fermentation extracts, that frequently, meaningful spectral data may be obtained on the separated bioactive component from a single counter-current chromatography. This paper outlines the use of CPC counter-current distribution in the isolation-separation of siderochelin A, efrotomycin, pentalenolactone, Bu 2313 B, and the closely related congeners, tirandamycins A and B. The discussion includes general methods for solvent system selection and sample preparation. Spectral data on the isolated compounds are presented as evidence for the quality of separations effected. The variety of structural types presented demonstrates the versatility of the coil planet centrifuge as a tool in separation science.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.