Determination Of Mexacarbate And Five Of Its Derivatives By High-Performance Liquid Chromatography

Abstract

A simple, sensitive and reliable analytical method has been developed and reported for mexacarbate (4-ditnethylamino-3,5-xylyl N-methylcarbamate) and five of its possible degradation products likely to be found in environmental samples using reversed-phase high-performance liquid chromatography with isocratic and gradient solvent systems. All chromatogram peaks were Identified through comparison to standards. The method has been used to identify and separate the six compounds from a mixture of the standards. It has been evaluated under different column conditions and with different mobile phases. Best resolution of the analytes was obtained by using a gradient solvent system consisting of CH₃CN and H₃O detecting at 200 nm and 30°C using a HP-RP8, 10 μ m, 20 cm 4.6 mm column.