Determination of 13 Catecholamines, Indoleamines, Metabolites and Precursors in Less Than 20 Minutes During a Single HPLC Run

H. G. Hadfield Neurochemistry Research Laboratory, Department of Pathology, and the Psychopharmacology Research Laboratory

P. Crane Clinical Chemistry Laboratory Department of Pathology, and the Psychopharmacology Research Laboratory

M. E. King Clinical Chemistry Laboratory Department of Pathology, and the Psychopharmacology Research Laboratory

E. A. Nugent Neurochemistry Research Laboratory, Department of Pathology, and the Psychopharmacology Research Laboratory

C. Milio Neurochemistry Research Laboratory, Department of Pathology, and the Psychopharmacology Research Laboratory

N. Narasimhachari Department of Psychiatry, Medical College of Virginia Virginia Commonwealth University, Richmond, Virginia, 23298

Abstract

Thirteen catecholamines and indoleamines are rapidly separated during a single HPLC run. They include epinephrine, norepinephrine, dopamine, serotonin, the major metabolites of these compounds and their immediate precursors. The technique was developed in order to analyze several biogenic amines in brain tissues simultaneously but the methodology has universal application. The separation is based on a fine balance between organic solvent, ion pairing reagent, buffers, pH, choice of column and flow rate.

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Determination of 13 Catecholamines, Indoleamines, Metabolites and Precursors in Less Than 20 Minutes During a Single HPLC Run

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Determination of 13 Catecholamines, Indoleamines, Metabolites and Precursors in Less Than 20 Minutes During a Single HPLC Run

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