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Abstract
Molecular weight distribution and the polydispersity of Heparins are important parameters for the characterisation and the standardisation of Heparin samples isolated from various raw materials as well as for correlations and ultimately predictions of biological activity toward thrombin. The combination of high pressure liquid chromatography (HPLC) with a low angle laser light scattering photometer has been applied to three standard heparins and a sodium and calcium salt of a particular heparin sample and is shown to be a rapid and accurate method for the determination of these parameters. The molecular weights of the standards thus determined are in good agreement with the results from classical light scattering and analytical ultracentrifuge measurements, while the data from various laboratories using conventional HPLC differ within a wide range.