Studies on Iodinated Compounds. IV. High Performance Liquid Chromatographic Determination of Iodide with a Complex of Primary Ammonium Ion - Crown Ether

Abstract

Determination of iodide (I⁻) was performed using a complex of primary ammonium ion - crown ether as the counter ion on reversed-phase high performance liquid chromatography (HPLC)_° Retention of the I⁻ in the present system increased more than the corresponding counter ion system, using the only primary ammonium ion_° The rate of increase of the retention was dependent on pHs', concentration of crown ether and organic solvents of the eluent. Determination of I⁻ was investigated with hexylamine and 18-crown-6 with a UV detector at 225 nm_° The calibration curve was linear from 25 pmoles to 25 nmoles, and the least detection of I⁻ was 1 pmole.