Use of Millipore Norganic Resin for the Extraction of Proctolin and Other Pharmacologically Active Constituents from Cockroach Tissue Homogenates

Abstract

Millipore Norganic resin, a proprietary product marketed for the preparation of HPLC-grade water, can serve as an alternative to Sep-Pak C₁₈ cartridges for the solid phase extraction of proctolin and other physiologically active components from cockroach tissue homogenates. For large scale isolations of such substances, this resin is most useful for first stage purification. Retained substances can be further purified by application to Sep-Pak C₁₈ cartridges which are less hydrophobic than the Norganic resin. Results were evaluated by reversed-phase HPLC of extracts and bioassay of fractions. Typical recoveries of proctolin were in the range of 75–90%.