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The application of chemically bonded chiral phases, prepared by binding various chelating ligands to silica gel via 3-glycidoxipropyltrimethoxysilane as spacer, for the separation of enantiomers, is described.
The influence of the nature of the matrix, the fixed ligand, the metal ion and the mobile phase on the enantioselectivity is discussed. Examples are given for the application of the system to the resolution of amino acids, amino acid derivatives, dipeptides and hydroxy acids.
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