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Abstract
The separation of cyclic nitros amines by HPLC was achieved using a cyclobond I column and a mobile phase of methanol/water. The effect of in-the-ring and on-the-ring substitutions on retention times were studied. The results show that the longer the chain of the substituent, the longer the retention time, which is consistent with the inclusion mechanism of separation. Also, the larger the ring, the longer the retention time. The results also show that the in-the-ring substitution has a dramatic effect on retention.