Combined Florisil, Droplet Counter-Current, and High Performance Liquid Chromatographies for the Preparative Isolation and Purification of Azadirachtin from Neem (Azadirachta Indica) Seeds

Abstract

An alternate prepurification procedure was developed for the isolation and purification of azadirachtin, a biologically active limonoid, from neem (Azadirachta indica) seeds by the use of Florisil and droplet counter-current chromatographies. The procedure, combined with the use of preparative high performance liquid chromatography, yielded 364.8 mg of >99% pure azadirachtin from 1.5 kg of Indian neem seeds.