A Microcomputer Program for the Calculation of R_F Values of Solutes in Stepwise Gradient Thin-Layer Chromatography

Abstract

A microcomputer program (in BASIC, e.g., for SPECTRUM ZX+) is proposed for the calculation of final R_F values obtained under conditions of stepwise gradient. After introduction of R_F values of sample components obtained for several isocratic runs, the microcomputer calculates the R_F values for any gradient program. Good agreement of calculated and experimental R_F values was obtained. The stepwise gradient development was carried out using a sandwich chamber with a glass distributor; the eluent fractions according to a chosen stepwise program were introduced directly under the distributor with a micropipette.