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Abstract
Rhodamine-123, a mitochondrial-specific dye, is currently of interest as a potential anticarcinoma agent. We now report an analytical method suitable for monitoring this agent in a variety of biological matrices. The drug is easily extractable into organic solvent in high yield (>90%). Analysis is conducted by reversed-phase HPLC using a phenyl column and an organic/aqueous buffer mobile phase, with signal detection by flow fluorometry. The assay shows linearity over the concentration range of 5-4000 ng/ml, with a limit of sensitivity of 100 pg. Preliminary animal data confirms the suitability of the assay for detailed drug metabolism and dispositional studies.