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Abstract
In the preparation of strigol, the last step of an 18-step synthesis produces strigol and its diastereomer, epistrigol. The two can be separated by column chromatography and recrystallization. It would be advantageous to be able to monitor the purity of each of these isomers in the presence of the other at each step of purification. Also, strigol is a much more potent witchweed seed germination stimulant than epistrigol and it is important to know the purity of samples submitted for bioassay. A satisfactory method was devised which utilized HPLC on a C-18 reverse phase column with a ternary solvent mixture of acetonitrile-methanol-water (10-50-40). The method gave good separation and reproducibility of analyses of mixtures of epistrigol and strigol and was sensitive enough to detect 1–2% of either isomer in the presence of the other.