Quantitative Analysis of Alkylacyl, Alkenylacyl, and Diacyl Types of Diglycerides Obtained from Glycerophospholipids

E. Francescangeli Institute of Biological Chemistry University of Perugia, 06100, Perugia, Italy; Department of Physiological Chemistry, The Ohio State University, Columbus, Ohio, 43210

S. Porcellati Institute of Biological Chemistry University of Perugia, 06100, Perugia, Italy; Department of Physiological Chemistry, The Ohio State University, Columbus, Ohio, 43210

L. A. Horrocks Institute of Biological Chemistry University of Perugia, 06100, Perugia, Italy; Department of Physiological Chemistry, The Ohio State University, Columbus, Ohio, 43210

G. Goracci Institute of Biological Chemistry University of Perugia, 06100, Perugia, Italy; Department of Physiological Chemistry, The Ohio State University, Columbus, Ohio, 43210

Abstract

The 1-alkyl-2-acyl, 1-alk-1'-enyl-2-acyl, and 1,2-diacyl-snglycerol moieties of choline and ethanolamine glycerophospholipids were converted to the dinitrobenzoyl derivatives. These compounds were separated by high performance liquid chromatography and quantitated by their absorbance at 235 nm. Peak areas were proportional to the amount injected. Separations were optimal for the derivatives from 5 to 30 nmol of the glycerophospholipids. The compositions of ethanolamine glycerophospholipids from rat brain and of choline and ethanolamine glycerophospholipids from human platelets agreed well with previous results obtained with much longer procedures.

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Quantitative Analysis of Alkylacyl, Alkenylacyl, and Diacyl Types of Diglycerides Obtained from Glycerophospholipids

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Quantitative Analysis of Alkylacyl, Alkenylacyl, and Diacyl Types of Diglycerides Obtained from Glycerophospholipids

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