HPLC Determination of Monoamines in Rat Brain after Enzymatic Treatment with Ascorbate Oxidase and Sulfatase

M. Gary Hadfield Neurochemistry Research Laboratory Department of Pathology, Medical College of Virginia Virginia Commonwealth University, Richmond, Virginia, 23298

Christine Milio Neurochemistry Research Laboratory Department of Pathology, Medical College of Virginia Virginia Commonwealth University, Richmond, Virginia, 23298

Abstract

The simultaneous analysis of norepinephrine, dopamine and serotonin along with their respective metabolites, MHPG, DOPAC and 5HIAA is accomplished in rat brain by using a recently developed HPLC technique. Sulfated MHPG is decongegated with sulfatase for detection purposes and endogenous ascorbate is diminished with ascorbate oxidase to reduce the front.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

HPLC Determination of Monoamines in Rat Brain after Enzymatic Treatment with Ascorbate Oxidase and Sulfatase

Information for

Open access

Opportunities

Help and information

HPLC Determination of Monoamines in Rat Brain after Enzymatic Treatment with Ascorbate Oxidase and Sulfatase

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature