Centrifugal Partition Chromatography. III. Physico-Chemical Properties of Ternary Liquid Systems

Abstract

Four important physico-chemical solvent parameters that affect centrifugal partition chromatography (CPC) were evaluated. They are (i) the mutual solubility of the solvent(s) in each phase which was analyzed using phase diagrams and tie-lines, the results of which can be used to evaluate the behavior and stability of the biphasic CPC system; (ii) solution density which affects the pressure drop; (iii) interfacial tension which affects droplet formation and (iv) viscosity which affects both pressure drop and chromatographic efficiency. These parameters were studied for three ternary solvent systems. The first one had a very low mutual solubility (water-hexane-octanol). The second one had an intermediate mutual solubility (water-hexane-methanol). The last one had a high mutual solubility (water-pentanol-methanol).