Detection of Trace Amounts of 8-Hydroxy-2′-Deoxyguanosine in Commercial 2′-Deoxyguanosine by Means of HPLC Analysis and Electrochemical Analysis

Abstract

A commercial sample of 2′-deoxyguanosine (dG) was submitted to reversed phase HPLC analysis. The column effluent was analyzed by UV detection set at 293 nm coupled to an electrochemical detector at 600 mV oxidation potential. When both detectors were set at their highest sensitivity, the UV detector did not show the presence of the 8-hydroxy-2′-deoxyguanosine but the electrochemical detector was capable of detecting this oxidatively modified 2′-deoxyguanosine. When planning in vitro experiments with 2′-deoxyguanosine in order to detect the oxidatively modified nucleoside it is necessary to assess the presence of this C8-hydroxylated derivative in the commercial product.