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Abstract
α-tocopherol acetate (Vitamin E-acetate) is extracted, separated and determined from pharmaceutical preparations and from biological materials in nanogram range in less than 25 minutes. The extraction of Vit. E-acetate is performed in a fully automated, electronically controlled extraction apparatus. A reversed phase high-performance liquid chromatography (HPLC) using a column of lichrosorb RP18 and methanol as eleuent has been developed for the separation and quantitative determination of Vit. E-acetate in formulations. The described extraction and determination methods are suitable for the analysis of Vit. E-acetat alone and in the presence of other water - and Fat-soluble Vitamins in pharmaceutical preparations and are reproducible with coefficient of variation of ∼3%. Vitamin A-acetate can be used as internal standard.