12
Views
6
CrossRef citations to date
0
Altmetric
Original Articles

Microbore Liquid Chromatography for Pediatric and Neonatal Therapeutic Drug Monitoring and Toxicology: Clinical Analysis of Chloramphenicol

, , , &
Pages 1143-1158 | Published online: 06 Dec 2006
 

Abstract

Microbore liquid chromatographic assay of chloramphenicol was developed, using 5 uL samples and reversed-phase liquid chromatographic analysis with a C-18, 20% carbon load column. For protein precipitation, serum samples were mixed with 20 uL of methanolic internal standard solutions. After centrifugation, 0.5 uL aliquots were injected for analysis. Two procedures were evaluated: procedure A-column = C-18, 3 um, carbon load of 10%, mobile phase = acetate/acetonitrile/tetrahydrofuran (85:15:1.5), flow rate = 80 uL/min, temperature = 50°C; procedure B-column = C-18, 5 um, carbon load of 20%, mobile phase = acetate/ acetonitrile(8:2), and flow rate = 60 uL/min. Procedure B was chosen for clinical efficacy study. The retention volumes of chloramphenicol and internal standard were 360 and 840 uL respectively. Calibration curve was linear between 3 to 40 mg/L. And day-to-day coefficient of variation was 6.8%. Correlation study with an established clinical liquid chromatographic assay for 20 patient samples showed acceptable data: r=0.9949, slope=0.9760 and intercept=0.0025. Selected drugs did not interfere. This assay, with advantages of small sample size, easy preparation and MBLC analysis, may be readily adapted for pediatric and neonatal drug monitoring.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.