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Abstract
A rapid, sensitive and selective normal phase chromatographic determination method is described for the determination of MTDQ/SensoradR/in human plasma. The drug was extracted into n-hexane-isoamylalcohol mixture (95, 5/o, 5 v/v) using a Partisil 5 μm chromatographic column, with n-hexane-methylenechloride-dioxane mixture (60/40/0,5 v/v) as eluent. Separation and quantification of MTDQ have been achieved within 15 min. The detection limit of the compound was 1 ng at 254 nm detection wavelength. The recovery was 90,4% and the coefficient of variation was found to be less than 4,5% in the concentration range of 0,01–10 μg/ml MTDQ in plasma. No drug, drug metabolite or endogenous substance interference was observed. Data on steady-state plasma concentration during long-term admonistration of the drug are presented. The effective concentration of MTDQ in plasma proved to be 1–7 μg/ml.
The reproducibility and precision of normal and reversed phase chromatography of the drug were compared.