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Special Section – Papers from the Second Colloquium on Centrifugal Partition Chromatography

Study of AcOH/HCOOH/H₂O/CHCL₃ Solvent System Application to the Separation of Two Large and Hydrophobic Fragments of Bacteriorhodopsin Membrane Protein by Centrifugal Partition Chromatography

Alain Foucault Department of Chemistry, Columbia University, New York, N.Y., 100027

Koji Nakanishi Department of Chemistry, Columbia University, New York, N.Y., 100027

Abstract

Centrifugal Partition Chromatography has been found useful for the separation of C₁ and C₂, two large hydrophobic peptides resulting from the chymotryptic cleavage of the membrane protein Bacteriorhodopsin. For this purpose the biphasic system AcOH/HCOOH/H₂O/CHCL₃ has been studied and the role of the two main parameters, i.e. Acetic acid and Formic acid, clearly demonstrated.

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Study of AcOH/HCOOH/H₂O/CHCL₃ Solvent System Application to the Separation of Two Large and Hydrophobic Fragments of Bacteriorhodopsin Membrane Protein by Centrifugal Partition Chromatography

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Study of AcOH/HCOOH/H2O/CHCL3 Solvent System Application to the Separation of Two Large and Hydrophobic Fragments of Bacteriorhodopsin Membrane Protein by Centrifugal Partition Chromatography

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Study of AcOH/HCOOH/H₂O/CHCL₃ Solvent System Application to the Separation of Two Large and Hydrophobic Fragments of Bacteriorhodopsin Membrane Protein by Centrifugal Partition Chromatography