Determination of Cefpiramide in Plasma by High-Performance Liquid Chromatographic with Internal Surface Reversed-Phase Silica Column

Abstract

We investigated the isolation of cefpiramide (CPM), cephem antibiotics, from human plasma proteins by high-performance liquid chromatography with a new column, Pinkerton^R column (Regis Chemical Co., Illinois, U.S.A.), and discussed whether the column is applicable to routine clinical determination. In condition of our studies, CPM could be analyzed without any pretreatment. The chromatograms of CPM obtained were single, sharp peaks. Proteins and endogenous compounds in plasma did not interfere with the assay. And, the precise free fraction of CPM could be obtained. The effect on the isolation by the different flow direction was also studied since the column could be used reversibly. There were significant differences (p < 0.01) between two slopes of the calibration curves by the different flow direction. It is suggested that calibration curves for both flow directions are required. The analytical procedures outlined in this study may be applicable to routine clinical use since this is a simple and rapid method.