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Abstract
A high performance liquid chromatographic method was developed for the determination of imipenem concentrations in plasma. This method involves stabilization of plasma with a mixture of 2-(N-morpholine)-ethanesulf onic acid and ethylene glycol (1:1). Samples are ultrafiltered using a membrane separation system and the ultrafiltrate injected directly onto a octyldecyl column. Chromatography is performed in the reverse-phase mode with a mobile phase of acetonitrile-phosphate buffer-triethylamine (pH 7.0). The lower limit of sensitivity was 1mcg/ml using UV detection at 300nm. Recovery and reproducibility results, and interferences from other therapeutic agents are presented and discussed. The assay procedure is applied to estimate pharmacokinetic parameters of imipenem in a thermally injured patient.