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Abstract
A simple method based on high-performance liquid chromatography with ultraviolet detection using octadecyl column is described for the simultaneous determination of norepinephrine, epinephrine and dopamine levels in mouse brain. Mouse brain was dissected into seven regions, striatum, hypothalamus, cerebellum, cerebral cortex, hippocampus, midbrain and medulla oblongata. The supernatant of a tissue homogenate was injected directly into a column, omitting the commonly performed adsorption step. The influence of pH, addition of acetonitrile and ion-pair reagents on chromatographic performance was studied. Assay sensitivities were sufficient for at least 20 pg of the above compounds in a mouse brain sample. The procedure is also readily applicable to determination of metabolites of catecholamines and serotonin levels.