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Abstract
The analysis of N-hydroxy-3,4-methylenedioxyamphetamine (NOHMDA) from plasma samples using reversed-phase liquid chromatography is described. The separation of NOHMDA, its metabolite 3,4-methylenedioxyamphetamine (MDA) and the internal standard N-methyl-MDA (MDMA) were well resolved in about twelve minutes using a C8 stationary phase and an acidic aqueous acetonitrile mobile phase. The isolation procedure involved liquid/liquid extraction of 50 uL of plasma using chloroform followed by HPLC analysis of the resulting residue. The recovery was greater than 90% for NOHMDA and slightly higher for MDA with good reproductibility.