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Original Articles

Corticosteroid Analysis by HPLC-UV Facilitated byv Use of an Injector-Mounted Extraction Column

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Pages 1257-1266 | Published online: 23 Oct 2006
 

Abstract

A method for integrating solid phase extraction of corticosteroids from biological samples with normal operation of reversed-phase HPLC is described. This method uses an extraction column mounted in place of the sample loop of a conventional injection valve to separate corticosteroids from some interfering compounds, and to effectively concentrate steroids from dilute samples. Both manual and fully automated chromatographs using this principle are described. A specific application to measurement of corticosterone in a rabbit serum preparation allows routine measurement of as little as 300 pg corticosterone per sample.

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