Silica Based 3,5-Dinitrobenzoyl (Dnb) Reagent for Off-Line Derivatization of Amine Nucleophiles in HPLC

Abstract

We describe here a new silica based derivatization reagent, containing the 3,5-dinitrobenzoyl tag, for solid phase derivatization of amines. It can be used for the off-line derivatization of primary and secondary amines. the amide derivatives can be easily detected under conventional UV detection modes. the entire synthetic method, structural characterization, and optimization of derivatization conditions of this solid phase derivatization reagent are described, Also, the reagent was tested in the on-line, pre-column derivatization mode for reversed phase HPLC, as well as for histamine analysis in fish samples