Optimization of the High-Performance Liquid Chromatographic Separation of Steroids by the Overlapping Resolution Mapping Procedure

Kiao Yuh Chong Department of Chemistry, National University of Singapore, Kent Ridge Republic

Teng Hai Khoo Department of Chemistry, National University of Singapore, Kent Ridge Republic

Fei Sheng Koo Department of Chemistry, National University of Singapore, Kent Ridge Republic

Chye Peng Ong Department of Chemistry, National University of Singapore, Kent Ridge Republic

Sam F. Y. Li Department of Chemistry, National University of Singapore, Kent Ridge Republic

Hian Kee Lee Department of Chemistry, National University of Singapore, Kent Ridge Republic

Byrappa Venkatesh Department of Zoology, National University of Singapore, Kent Ridge Republic

Cheong Huat Tan Department of Zoology, National University of Singapore, Kent Ridge Republic

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Abstract

The reversed-phase high-performance liquid chromatographic separation of several steroids is described in this paper. The optimization of the solvent composition was achieved by making use of the overlapping resolution mapping scheme. The optimum solvent composition was arrived at from only seven chromatographic experiments. Satisfactory separation was possible for all but one pair of the steroids considered.

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Optimization of the High-Performance Liquid Chromatographic Separation of Steroids by the Overlapping Resolution Mapping Procedure

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Optimization of the High-Performance Liquid Chromatographic Separation of Steroids by the Overlapping Resolution Mapping Procedure

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