Matrix Solid Phase Dispersion (MSPD) Isolation and Liquid Chromatographic Determination of Clorsulon in Milk

Abstract

A method for the isolation and liquid chromatographic determination of the anti-parasitic drug clorsulon in milk is presented. Milk samples (0.5 g) were blended with 2 grams of C-18 (octadecylsilyl-derivatized silica column packing material). A column was made from the C-18/milk matrix and first washed with hexane. The clorsulon was then eluted from this column with ethyl ether. The ether eluate was cleaned up by Florisil solid phase extraction, and clorsulon was subsequently determined by liquid chromatography with UV detection. The overall recovery of clorsulon from milk spiked at 50–200 ppm was 93.0&with a coefficient of variation of 5.6 & The MSPD method and a solid phase extraction method used by the FDA gave comparable values for milk containing incurred clorsulon residues.