Interest of a Simple Mobile Phase Containing Diethylamine-Acetic Acid for HPLC Assay of Nicotine and Cotinine

D. Roche Laboratoire Central de BiochemieHôpital Laënnec, 42 rue de Sèvres, 75007, Paris, France

I. Toury Laboratoire Central de BiochemieHôpital Laënnec, 42 rue de Sèvres, 75007, Paris, France

N. T. Lequang Laboratoire Central de BiochemieHôpital Laënnec, 42 rue de Sèvres, 75007, Paris, France

G. Roussel Centre de Prévention des Maladies Respiratoires, Consultation Anti-Tabac Hôpital Laënnec, 42 rue de Sèvres, 75007, Paris, France

O. G. Ekindjian Laboratoire Central de BiochemieHôpital Laënnec, 42 rue de Sèvres, 75007, Paris, France

Abstract

We have developed a simple mobile phase containing a diethylamine/acetic acid mixture free of salts and ion-pairing compounds for the assay of nicotine and cotinine in biological media by means of high-performance liquid chromatography. The best composition was found to be methanol/acetonitrile/acetic acid/water (230/20/2/748 v/v) adjusted to pH 5.00 or 6.40 with diethylamine. The results were excellent when compared with those obtained from

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Interest of a Simple Mobile Phase Containing Diethylamine-Acetic Acid for HPLC Assay of Nicotine and Cotinine

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Interest of a Simple Mobile Phase Containing Diethylamine-Acetic Acid for HPLC Assay of Nicotine and Cotinine

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