Comparison of Different Compositions of Mixed Pentane/1,4-Dioxane Mobile Phases in SFC

Abstract

The effective plate number has been chosen for a comparison between different mixed mobile phases composed of pentane and 1,4-dioxane. A column packed with unmodified silica gel and pyrene as the analyte was used. The dependence of the effective plate number on pressure and composition for a constant temperature, or a constant reduced temperature, both at constant mass flow, are shown in three dimensional network plots. The same dependence is shown at constant temperature and constant linear velocity.

For a detailed discussion, the dependence of the effective plate number on pressure, composition, capacity ratio, and other chromatographic parameters is given in two dimensional graphs. The main result for the present chromatographic system is that a Financial support by the Arbeitsgemeinschaft Industrieller Forschungsvereinigungen (AIF) is gratefully acknowledged.