![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
Utilizing retention data estimated by HPTLC on silica and activity coefficients in the non-aqueous mobile phase determined on the basis of saturation solubility of solutes, the effect of the mobile phase on retention of a set of structurally different solutes was studied. A quadratic relationship between the logarithm of retention factors or activity coefficients and the volume fraction of ethyl acetate in heptane - ethyl acetate solution was observed, suggesting a common retention machanism in liquid chromatography. The retention and/or relative retention of a solute was affected by both the mobile and the stationary phase. The magnitude of these effects depended merely on the molecular structure of a solute.