Comparison of Mobile Phases for Separation of Phospholipids by One-Dimensional TLC on Preadsorbent High Performance Silica Gel Plates

Abstract

Eight solvent systems reported in the literature for the one-dimensional TLC separation of phospholipids were compared under identical conditions using high performance preadsorbent silica gel plates. The best overall separation of phospholipid standards was obtained by a single development with chloroform-methanol-water (65:25:4), and three other systems containing chloroform also gave good separations. Rf data are tabulated for these four systems, and the phospholipids extracted from the digestive gland-gonad complex of Biomphalaria glabrata snails are identified.