Reversed-Liquid Phase Chromatographic Determination of Cefpodoxime in Human Plasma

Abstract

The reversed-phase liquid chromatographic determination of cefpodoxime in human plasma is described. Plasma samples were acidified with 1 mL of phosphoric acid solution containing internal standard, and adsorbed onto a C8 solid phase cassette designed specifically for use with a Varian Advanced Automated Sample Processor. Each cartridge on the cassette was washed with 1 mL of phosphoric acid containing 5% methanol and then was placed into the automated sample processor for direct elution of the retained drugs into the chromatographic system. Chromato-graphy was free from interferences in the analyte and internal standard windows, and yielded excellent linearity, accuracy, and precision in studies performed in this laboratory.