Interaction of Diquat and Paraquat with Glutathione Studied by Means of Charge-Transfer Chromatography

Abstract

The interaction of reduced and oxidized glutathiones with the herbicides diquat and paraquat was studied by charge-transfer chromatography carried out on un-impregnated cellulose layers. Diquat and paraquat interact both with reduced and oxidized glutathiones forming charge-transfer complexes. The interaction is of hydrophilic character in both cases. The formation of covalently bonded conjugates was not observed. The strength of interaction decreases in ionic (LiCl, NaCl, KCl, MgCl₂ and CaCl₂) environment in the case of oxidized glutathione. The charge of cation had a higher impact on the strength of interaction than that of the hydrated ion radii.