Large Scale Purification of Immunregulatory Peptide N^α 1, N^∊2- Diacetyl-Splenopentin by Chromatography on a Carboxylated Cation Exchanger

Abstract

The immunregulatory pentapeptide ARG-LYS-GLU-VAL-TYR was synthesized by using the mixed anhydride coupling method. Acylation of N^α1- and N^∊2-amino groups increases the enzymatic degradation stability of splenopentin. This paper describes a simple and effective large scale purification method of N^α1-, N^∊2-diacetyl-splenopentin synthesis product by chromatography on the carboxylated cation exchanger Wofatit Y 79. Using ethanol/acidic water as eluent the isolation of purified peptide from the eluat was achieved directly by evaporation under vacuum and lyophilisation.