Abstract
The paper describes the technique of solvent-generated liquid-solid chromatography with a chiral stationary phase consisting of permethylated β-cyclodextrin coated on a bare silica surface. The chiral adsorption layer was generated dynamically by pumping diluted solutions of permethylated β-cyclodextrin in binary (hexane/different alcohols) eluents through a column packed with microparticulate silica. This technique leads to column with excellent time stability and good reproducibility of the enantioselectivity. It was found that retention characteristics e. g. enantioselectivity and capacity depend very strongly on the type and concentration of the alcohol used as mobile phase component.