Isocratic HPLC Separation of Scopoletin and Cis/Trans Isomers of Ferulic Acid as Well as Isoscopoletin and Cis/Trans Isomers of Isoferulic Acid

M. Fernanda M. Borges Laboratório de Química Org[acaron]nica Faculdade de Farmácia do Porto Rua Aníbal Cunha, 4000, Porto, Portugal

Fernanda M. F. Roleira Laboratório de Química Org[acaron]nica Faculdade de Farmácia do Porto Rua Aníbal Cunha, 4000, Porto, Portugal

Madalena M. M. Pinto Laboratório de Química Org[acaron]nica Faculdade de Farmácia do Porto Rua Aníbal Cunha, 4000, Porto, Portugal

Abstract

Cis/trans isomers of ferulic and isoferulic acids and their corresponding coumarins, scopoletin and isoscopoletin, were separated by isocratic High Performance Liquid Chromatography using RP-8 (5 μm) as a stationary phase and aqueous methanol or aqueous acetonitrile as a mobile phase. The UV spectrum of cis-isoferulic acid was obtained by a photodiode array detector.

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Isocratic HPLC Separation of Scopoletin and Cis/Trans Isomers of Ferulic Acid as Well as Isoscopoletin and Cis/Trans Isomers of Isoferulic Acid

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Isocratic HPLC Separation of Scopoletin and Cis/Trans Isomers of Ferulic Acid as Well as Isoscopoletin and Cis/Trans Isomers of Isoferulic Acid

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