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Abstract
The present study was conducted to examine the presence of insulin-like growth factor-1 receptor (IGF1R) in human sperm cell. The IGF-1R monoclonal antibody (MAb) specifically immunoprecipitated two protein bands of 135 and 220 kD, respectively, from the reduced human sperm extract. These bands correspond to the alpha-subunit and partially reduced IGF-1R molecule, respectively. The immunocytochemistry experiments indicated the presence of IGF-1R primarily in the equatorial regions, along with a weak binding in the acrosomal regions of human spermatozoa. These data indicate that the IGF1 receptor is present in the human sperm cell. Since the IGF-1 receptor has tyrosine kinase activity and its ligand IGF-1 is present in the seminal plasma, the IGF-1 system (IGF-1R/IGF-1/IGF-binding protein) may be involved in the signal transduction pathway leading to sperm capacitation and acrosome exocytosis.