Isolation, Identification and Efficacy of Inorganic Phosphate-Solubilizing Bacteria from Oxbow Lakes of West Bengal, India

Abstract

Microbial mobilization of sediment calcium-bound P constitutes an important process of P cycling in aquatic environments. The present study was conducted to identify the bacterial community responsible for inorganic phosphate solubilization in tropical oxbow lakes. Fifty eight phosphate-solubilizing bacteria were isolated from bottom soil, water, and fish gut and examined for solubilization of tricalcium phosphate. Results revealed aquatic PSB to be low-to-moderately capable in P solubilization (mean: 33.5 mg P L⁻¹; range: 6.3–68.8 mg P L⁻¹), and bacteria from wetland sediment and water were more effective than those from fish gut. The PSB were identified to belong to diverse genera, viz. Bacillus, Brevibacillus, Enterobacter, Agrobacterium, Pseudomonas, Acinetobacter, Microbacterium, Curtobacterium, Stenotrophomonas and Novosphingobium. The findings help in understanding the microbial role in inorganic P solubilization and identifying important P solubilizers in freshwater environments.

Keywords:

• Ca-P
• India
• PSB
• oxbow lakes
• solubilization

Funding

The work was supported by ICAR funded AMAAS Network project on “Microbial Phosphorus Transformations in Inland Open Waters.”