Abstract
Calcium carbonate precipitation in the natural environment is associated with a variety of factors including type species of bacteria. In this study, we investigated calcium carbonate precipitation induced by bacteria isolated from rocks of Murree Hills, Lower Himalayan region, Pakistan. Four species; Acinetobacter N1, Acinetobacter N6, Bordetella N30, and Brevundimonas N5, having ureolytic activity, were found to precipitate calcium carbonate. FTIR analysis revealed the formation of calcium carbonate crystals confirmed by comparison to synthetically produced calcium carbonate. Morphological characterization by scanning electron microscopy showed crystals formed were mostly of the irregular and polygonal shape having sharp spike-like edges. All the four strains having urease enzymatic capability to convert urea into ammonia to produce calcium carbonate crystals in higher pH due to the production of an alkaline environment. The results of our research revealed a variety of rock bacteria capable of precipitating calcium carbonate in natural environments and are expected to have a great influence on the cycling of carbonates in these environments.
Disclosure statement
No potential conflict of interest was reported by the author(s).