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Geomicrobiology Journal Volume 7, 1989 - Issue 3
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Original Articles

Possible nonanthropogenic origin of two methanogenic isolates from oil‐producing wells in the san miguelito field, ventura county, California

Thomas K. Ng Central Research and Development Department, E.I. DuPont de Nemours and Co., Inc., Experimental Station, Wilmington, DE, 19898; Production Research Division, Conoco, Inc., Ponca City, OK, 74603
,
Paul J. Weimer Central Research and Development Department, E.I. DuPont de Nemours and Co., Inc., Experimental Station, Wilmington, DE, 19898
&
Len J. Gawel Genelabs, Inc., 505 Penobscot Drive, Redwood City, CA, 94063
Pages 185-192 | Received 15 May 1989, Published online: 28 Jan 2009
 
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Abstract

Two novel strains of rod‐shaped methanogens were isolated from oil‐producing wells of high temperature and moderate salinity in the San Miguelito field. The strains grow at 65°C in media containing 6% NaCl, form large aggregates of cell materials in liquid culture, and produce methane from H2 and CO2 only. The geochemistry and microbiology of the oil reservoir and surrounding areas support the view that not all methanogens isolated from oil wells are results of colonization during or after human exploration, and that methanogens can exist in situ under thermal, haline conditions.

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